Conventional temperature and humidity chambers are widely used in electronics, plastics, automotive, and aerospace industries to simulate environmental conditions such as heat, cold, and varying humidity. They are essential for evaluating product reliability, identifying potential defects, and ensuring compliance with quality standards.
However, in more advanced research and testing fields—such as biological studies, pharmaceutical research, packaging evaluation, and corrosion testing—temperature and humidity alone are not sufficient. Many biological and material processes are highly sensitive to atmospheric composition, especially CO₂ concentration, which affects chemical reactions, cell metabolism, and product stability.
LIB industry CO₂ temperature humidity chambers provide precise control over temperature, relative humidity (RH), and CO₂ concentration, allowing researchers and engineers to reproduce real-world environmental conditions with exceptional accuracy and reproducibility.
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A CO₂ temperature humidity chamber is an advanced environmental simulation tool designed on the basis of a conventional temperature and climatic test chamber but enhanced with CO₂ gas control. These CO₂ climatic chambers combine heating, cooling, humidification, and gas regulation systems to maintain a controlled environment for biological, material, and packaging testing.
Key specifications and features include:
• Temperature range: -60°C to +150°C (customizable high/low temperature options available),with ±0.5°C temperature uniformity
• Humidity range: 10% RH to 98% RH, with ± 1% RH~± 1.5% RH uniformity
• CO₂ concentration control: 0–20% (adjustable for specialized applications),with <± 0.4% RH CO₂ accuracy
• Workroom volume: 50L–1000L,standard and walk-in types depending on application.
• Key components: PID-controlled heating/cooling system, ultrasonic or steam humidification, CO₂ gas sensor, flow regulator, and safety exhaust system
In biological and life science applications, CO₂ is crucial for maintaining the pH balance of culture media, supporting cell metabolism, and ensuring healthy microbial growth. For example, mammalian cell cultures typically require 5% CO₂ at 37°C with high humidity to simulate the human physiological environment.
• Cell and tissue incubation
• Microbial growth studies
• Drug stability and pharmaceutical testing
• Regenerative medicine and stem cell research
• Maintains stable pH in bicarbonate-buffered media
• Supports long-term cell proliferation without manual intervention
• Prevents contamination through airtight and filtered air circulation
• Enables programmable temperature and CO₂ cycles to simulate diurnal or metabolic variations
Standards often referenced in biological testing include ISO 13485 for medical devices and USP <71> and <797> for sterile preparations, which highlight the importance of controlled environmental conditions for reproducibility and regulatory compliance.
In material science and corrosion testing, the presence of CO₂ significantly accelerates chemical reactions. When CO₂ combines with water vapor, it forms carbonic acid (H₂CO₃), which promotes surface oxidation and material degradation.
Typical applications include:
• Metal corrosion evaluation under CO₂-rich atmospheres
• Coating and paint durability testing
• Packaging material stability in modified atmosphere packaging (MAP)
• Battery enclosure and electronics housing testing
Example testing standards include ASTM D1735 / ISO 9227 for corrosion and salt spray simulation, ASTM F2139 for modified atmosphere packaging, and CO₂ exposure combined with temperature/humidity cycles to simulate accelerated aging.
LIB Industry also offers a comprehensive range of environmental corrosion chambers for various industrial and research applications.
LIB Industry Comprehensive Corrosion & Environmental Test Chambers | |
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This expanded portfolio enables researchers and engineers to simulate real-world corrosive and mixed-gas environments, providing reliable data for product development, durability testing, and material optimization.
LIB Industry CO₂ temperature and humidity chambers are designed for precision, reliability, and safety, ensuring optimal testing outcomes.
Key advantages include:
• High-precision control: PID regulation maintains CO₂, temperature, and humidity within ±0.5°C/±0.1% CO₂
• Flexible customization: Multiple chamber sizes (50L–1000L) and CO₂ concentration ranges to suit specific industry needs
• Enhanced safety: Integrated CO₂ leakage detector, auto ventilation system, overpressure protection, and emergency shutoff
• Smart interface: Touchscreen controller with data logging, remote monitoring, alarm notifications, and automated test programming
• Reliable performance: Designed for continuous 24/7 operation, suitable for long-term incubation or corrosion tests
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Application Field | Testing Purpose | Recommended Setting Example | Standards Referenced |
Biotechnology | Cell / tissue culture | 37°C, 95% RH, 5% CO₂ | ISO 13485, USP <71> |
Food & Packaging | Modified atmosphere storage test | 25°C, 80% RH, 10% CO₂ | ASTM F2139, ISO 22000 |
Material Research | CO₂ corrosion and aging | 50°C, 90% RH, 15% CO₂ | ASTM D1735, ISO 9227 |
Battery & Electronics | Gas leakage and sealing study | 30°C, 60% RH, 5% CO₂ | IEC 62133, UL 2054 |
CO₂ temperature humidity chambers represent a new generation of environmental simulation technology. They allow precise control of temperature, humidity, and CO₂, enabling accurate and reproducible results for biological incubation, corrosion analysis, material testing, and packaging evaluation.
✅ 3-Year Warranty & Lifetime Technical Support
⚙️ One-stop Testing Solutions & Full Customization Options
⚡ 1–3 Hour Rapid Response to Requests
7–15 Days Fast Delivery
72-Hour Continuous Pre-Shipment Testing
With over 16 years of expertise in environmental simulation equipment, LIB Industry ensures reliability, quality, and responsiveness from initial inquiry to installation.
Contact LIB Industry today inquiry@libtestchamber.com to explore our CO₂ environmental test chambers and discover how our tailored solutions can enhance your biological, corrosion, and material testing performance.
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